منابع مشابه
Time-Lapse Microscopy Imaging
INTRODUCTION By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex syst...
متن کاملTime-Lapse Imaging
By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex systems, but thei...
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Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine...
متن کاملIn vivo time-lapse imaging of neuronal development in Xenopus.
In vivo fluorescence imaging of cells in the developing nervous system is greatly facilitated in specimens in which cells are brightly but sparsely labeled. In this article, we describe a number of techniques that can be used for delivering fluorophore to neurons in the albino Xenopus laevis tadpole. Fluorescent dye or DNA that encodes a fluorescent protein can be delivered to single cells by e...
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Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments--including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes--that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being s...
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ژورنال
عنوان ژورنال: Eos, Transactions American Geophysical Union
سال: 2014
ISSN: 0096-3941
DOI: 10.1002/2014eo460001